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What Is Fluorescence Microscopy? Fluorescence microscopy is a type of optical microscopy that uses fluorescence to study the properties of biological and non-biological specimens. In this imaging technique, specimens are labeled with fluorescent dyes or proteins that emit light of a specific color when illuminated with light of a different color. This emitted light is then detected and used to cre …

Microscope Contrast Techniques When using a microscope with brightfield, some samples will have a natural contrast that is easily viewed, such as bright plants and flowers, metals, and pigments. Some samples can be stained to increase the microscope contrast. However, low contrast samples such as unstained bacteria, thin tissue slices, live cells, or reflective metal parts require the use of addit …